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Step 4

Prepare workspace

4

Prepare workspace

Prepare and sterilize your workspace before the next phase.

Instructions

Protective Gear

Before prepping your workspace, put on clean clothes, gloves, and a face mask. Spray your hands/gloves with 70% alcohol before touching anything or placing them in your still-air environment.

Face mask and disposable gloves Face mask and disposable gloves

Sanitizing your workspace

Spray your work area with 70% alcohol and allow it to dry. Alcohol needs at least 30 seconds to sit before it can effectively disinfect a surface. Allowing it to dry instead of wiping it will give the alcohol sufficient time to do its job.

Spray bottle and rubbing alcohol Spray bottle and rubbing alcohol

Killing Spores

If you've worked with spores or want to be safe, spray the surface (after it's dry) with 5-30% hydrogen peroxide. Allow it to dry or leave on the surface for at least 10 minutes.

Spray bottle and hydrogen peroxide Spray bottle and hydrogen peroxide

Sanitizing your tools

Spray any tools that will be added to your work area with 70% alcohol before placing them there. Allow them to air dry.

Tools and spray bottle filled with alcohol Tools and spray bottle filled with alcohol

Allow the area to settle

Once everything has been sanitized and placed within the work area, allow things to settle for at least 15 minutes before doing any work.

4

You've Completed Step 4

Next Up

5. Remove agar from pressure cooker

Remove your sterilized agar from the pressure cooker.

Start Step 5