Creating Potato Dextrose Agar (PDA)

Potato Dextrose Agar is a nutrient-rich medium used for cultivating fungi. Follow this guide to create your own batch for your next culture.

Estimated Time: 4 hr 30 min

Total Steps: 7

Guide Materials

This is the full list of materials needed for this guide. Some of these items may be optional or interchangeable.

Parafilm

$32.00 | Amazon

Plastic Gloves

$8.00 | Amazon

Face Mask

$25.00 | Amazon

12% Hydrogen Peroxide

$24.00 | Amazon

70% isopropyl alcohol

$7.00 | Amazon

Petroleum Jelly

$5.50 | Amazon

Pressure Cooker

$500.00 | Amazon

Petri Dishes : 20

$25.00 | Amazon

Digital Kitchen Scale

$12.00 | Amazon

Erlenmeyer Flask - 1000ml

$21.00 | Amazon

Poly-fil

$34.00 | Amazon

Dextrose : 10g

$15.00 | Amazon

Agar Powder : 10g

$32.00 | Amazon

Cheesecloth

$13.00 | Amazon

Unpeeled Potatoes : 160g

No link available

Boiling Pot

No link available

Aluminum Foil

No link available

Paper Towels

No link available

Ready to get started?

Now that you've been introduced to the course, let's dive in.

Next Up

1. Mixing potato water

Mix your potato water with agar and dextrose.